A RAPID METHOD FOR THE DETERMINATION OF BACTERIAL FATTY-ACID COMPOSITION

被引：33

作者：

ROZES, N ^{[1
]}

GARBAY, S ^{[1
]}

DENAYROLLES, M ^{[1
]}

LONVAUDFUNEL, A ^{[1
]}

机构：

[1] UNIV BORDEAUX 2,INST OENOL,351 COURS LIBERAT,F-33405 TALENCE,FRANCE

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1993年 / 17卷 / 03期

关键词：

D O I：

10.1111/j.1472-765X.1993.tb01440.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fatty acid methyl esters suitable for gas chromatographic analysis were prepared by treatment of bacterial cells with sodium methoxide (1 N) in anhydrous methanol. The methanolysis of cells, washed or not with sterile water, was carried out at room temperature for 5 min. This method was effective for Gram-positive and Gram-negative bacteria, but not for growing yeast cells. The coefficient of variation of fatty acid methyl ester values was 4.7% of the mean values (range 0.4-25%).

引用

页码：126 / 131

页数：6

共 20 条

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