THE PDHR-ACEEF-IPD OPERON OF ESCHERICHIA-COLI EXPRESSES THE PYRUVATE-DEHYDROGENASE COMPLEX

被引：99

作者：

QUAIL, MA ^{[1
]}

HAYDON, DJ ^{[1
]}

GUEST, JR ^{[1
]}

机构：

[1] UNIV SHEFFIELD, DEPT MOLEC BIOL & BIOTECHNOL, KREBS INST, SHEFFIELD S10 2UH, ENGLAND

来源：

MOLECULAR MICROBIOLOGY | 1994年 / 12卷 / 01期

基金：

英国惠康基金;

关键词：

D O I：

10.1111/j.1365-2958.1994.tb00998.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcript mapping and studies with lacZ translational fusions have shown that the pdhR gene (formerly genA) is the proximal gene of the pdhR-aceE-aceF-lpd operon encoding the pyruvate dehydrogenase (PDH) complex of Escherichia coli. A pdhR-lpd read-through transcript (7.4 kb) initiating at the pyruvate-inducible pdh promoter, and a smaller lpd transcript (1.7 kb) initiating at the independent lpd promoter, were identified. Evidence showing that the pdhR gene product negatively regulates the synthesis of the PdhR protein and the PDH complex via the pdh promoter was obtained, with pyruvate (or a derivative) serving as the putative inducing coeffector. The partially purified PdhR protein was also found to specifically retard and protect DNA fragments containing the pdh promoter region. The pdh promoter was not strongly controlled by ArcA, FNR or CRP.

引用

页码：95 / 104

页数：10

共 40 条

[1] OVEREXPRESSION AND SITE-DIRECTED MUTAGENESIS OF THE SUCCINYL-COA SYNTHETASE OF ESCHERICHIA-COLI AND NUCLEOTIDE-SEQUENCE OF A GENE (G30) THAT IS ADJACENT TO THE SUC OPERON [J].

BUCK, D ;

GUEST, JR .

BIOCHEMICAL JOURNAL, 1989, 260 (03) :737-747

[2] POSITIONAL REQUIREMENTS FOR THE FUNCTION OF NIF-SPECIFIC UPSTREAM ACTIVATOR SEQUENCES [J].

BUCK, M ;

WOODCOCK, J ;

CANNON, W ;

MITCHENALL, L ;

DRUMMOND, M .

MOLECULAR & GENERAL GENETICS, 1987, 210 (01) :140-144

[3] REGULATION OF PYRUVATE DEHYDROGENASE COMPLEX SYNTHESIS IN ESCHERICHIA-COLI KL2 - IDENTIFICATION OF INDUCING METABOLITE [J].

DIETRICH, J ;

HENNING, U .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1970, 14 (02) :258-+

[4] IDENTIFICATION AND SEQUENCING OF THE ESCHERICHIA-COLI CET GENE WHICH CODES FOR AN INNER MEMBRANE-PROTEIN, MUTATION OF WHICH CAUSES TOLERANCE TO COLICIN-E2 [J].

DRURY, LS ;

BUXTON, RS .

MOLECULAR MICROBIOLOGY, 1988, 2 (01) :109-119

[5] SYNTHESIS OF E-COLI PYRUVATE DEHYDROGENASE COMPLEX - NON-DEPENDENCE ON 3'-5'-CYCLIC AMP [J].

ENGELRAE, M ;

HENNING, U .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1973, 53 (03) :812-817

[6] THE ROLE OF INTEGRATION HOST FACTOR IN GENE-EXPRESSION IN ESCHERICHIA-COLI [J].

FREUNDLICH, M ;

RAMANI, N ;

MATHEW, E ;

SIRKO, A ;

TSUI, P .

MOLECULAR MICROBIOLOGY, 1992, 6 (18) :2557-2563

[7]

FUJITA Y, 1989, J BIOL CHEM, V264, P4201

[8] MESSENGER-RNA ANALYSIS OF THE ADC GENE REGION OF CLOSTRIDIUM-ACETOBUTYLICUM DURING THE SHIFT TO SOLVENTOGENESIS [J].

GERISCHER, U ;

DURRE, P .

JOURNAL OF BACTERIOLOGY, 1992, 174 (02) :426-433

[9]

GUEST JR, 1983, J GEN MICROBIOL, V129, P671

[10]

GUEST JR, 1989, ANN N Y ACAD SCI, V573, P76

← 1 2 3 4 →