DETECTION OF FETAL CELLS IN MATERNAL BLOOD

被引：48

作者：

YEOH, SC

SARGENT, IL

REDMAN, CWG

WORDSWORTH, BP

THEIN, SL

机构：

[1] UNIV OXFORD,JOHN RADCLIFFE HOSP,NUFFIELD DEPT OBSTET & GYNAECOL,HARRIS BIRTHRIGHT PERINATAL UNIT,OXFORD OX3 9DU,ENGLAND

[2] UNIV OXFORD,JOHN RADCLIFFE HOSP,INST MOLEC MED,MOLEC IMMUNOL UNIT,OXFORD OX3 9DU,ENGLAND

[3] UNIV OXFORD,JOHN RADCLIFFE HOSP,INST MOLEC MED,MOLEC HAEMATOL UNIT,OXFORD OX3 9DU,ENGLAND

来源：

PRENATAL DIAGNOSIS | 1991年 / 11卷 / 02期

基金：

英国惠康基金;

关键词：

PRENATAL DIAGNOSIS; ENZYMATIC AMPLIFICATION; POLYMERASE CHAIN REACTION; FETAL CELLS; FLOW CYTOMETRY;

D O I：

10.1002/pd.1970110207

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report the detection of fetal cells in the maternal circulation by enzymatic amplification of a single copy gene sequence that was fetal-specific. Fetal HLA-A2-positive cells were sorted from maternal HLA-A2-negative cells by flow cytometry and confirmed by demonstration of a fetal-specific HLA-DR4 sequence. However, this sequence could not be detected in unenriched maternal DNA prepared at 28 and 32 weeks' gestation. The sensitivity of detection was 1 HLA-DR4-positive cell in 10(5) HLA-DR4-negative cells. We conclude that prenatal diagnosis of paternally inherited autosomal-dominant genetic defects may be possible by selective gene amplification of maternal peripheral blood. However, preliminary enrichment for fetal cells may be necessary.

引用

页码：117 / 123

页数：7

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