PARA-NITROPHENYLLAURATE - A SUBSTRATE FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF LIPASE ACTIVITY

被引：19

作者：

MAURICH, V

ZACCHIGNA, M

PITOTTI, A

机构：

[1] UNIV UDINE,INST ALIMENTARY TECHNOL,VIA MARANGONI 97,I-33100 UDINE,ITALY

[2] UNIV TRIESTE,DEPT PHARMACEUT SCI,I-34100 TRIESTE,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 566卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80262-B

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Many assay procedures have been devised to measure lipolytic activity, but none is without problems. It is for this reason that new methods are still being proposed. In this work we have investigated the use of two esters of p-nitrophenol, the palmitic acid and lauric acid esters, as substrates for a highly sensitive high-performance liquid chromatographic method. Data on recovery, specific activity and reproducibility are reported only for the lauric ester, because the palnitic ester turned out to be a very poor substrate.

引用

页码：453 / 459

页数：7

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