PURIFICATION AND CHARACTERIZATION OF PISUM SEED TRYPSIN-INHIBITORS

被引：46

作者：

DOMONEY, C ^{[1
]}

WELHAM, T ^{[1
]}

SIDEBOTTOM, C ^{[1
]}

机构：

[1] UNILEVER RES LABS, SHARNBROOK MK44 1LQ, BEDS, ENGLAND

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 1993年 / 44卷 / 261期

关键词：

PISUM-TRYPSIN INHIBITOR; TRYPSIN-LIKE PROTEASE;

D O I：

10.1093/jxb/44.4.701

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Seed trypsin inhibitors have been purified from Pisum. Three groups of inhibitor were separated using column chromatography, whereas non-denaturing activity gels showed that five inhibitors could be distinguished. N-terminal sequence data obtained for two purified inhibitors showed that these belong to the Bowman-Birk class of inhibitors. Partial purification and characterization of a protease with in vitro trypsin-like activity from Pisum seed protein preparations indicated that this endogenous protease is not inhibited by any of the fractionated inhibitors.

引用

页码：701 / 709

页数：9

共 39 条

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[2]

ARENTOFT AM, 1991, ASPECTS APPL BIOL, V27, P211

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