AN ANALYTIC METHOD TO EVALUATE DOUBLE LABELLING EXPERIMENTS USING C14- AND H3-THYMIDINE FOR EXPONENTIAL GROWTH

The paper describes an analytic method for the evaluation of experiments by using the double labelling technique (application of C-14- and H-3-thymidine) with exponentially growing cell populations. By the analytic method it is possible to calculate exactly the DNA synthesis time and generation time, if the duration of (G+M) is approximately known. Uncertainty of knowledge of the value for (G+M) is only of minimal influence on the results. Thus it is possible to use beside the method of labelled mitoses the double labelling technique as a second method to determine the cell cycle for exponential growth. © 1968 Springer-Verlag.