TEMPERATURE-SENSITIVE PERIODS AND AUTONOMY OF PLEIOTROPIC EFFECTS OF L(1)NTS1, A CONDITIONAL NOTCH LETHAL IN DROSOPHILA

Analysis of temperature-shift experiments using strains homo- and/or hemizygous for a temperature-sensitive (ts) mutation of the Notch locus, l(1)Nts1, has permitted us to localize temperature-sensitive periods (TSPs) both for lethality and for adult ectodermal morphology defects. Discrete TSPs for lethality are localized to the first half of the embryonic period, to the second larval instar, to the third larval instar, and to a 15 hr period immediately after pupation. TSPs for adult morphology defects are localized to the second and third larval instars for eyeless-headless and duplicated antenna, to the third larval instar for small and rough (spl-like) eye, eye scar, fused leg segments, shortened tarsal leg segments, Notch wings, and extra macrochaetae, and to the early pupal period for extra and missing microchaetae, fag-like rough eye and thick wing vein defects. Within the third larval instar, distinct patterns of eye, wing, and leg defects are observed. There is a striking similarity between the adult morphology defects and TSPs of l(1)Nts1 and those of the larval and adult locomotor mutant, shits1 (C. A. Poodry, L. Hall, and D. T. Suzuki, 1973, Develop. Biol. 32, 373-386). Expression of l(1)Nts1 also has been studied in genetic mosaics, in which we find that the pleiotropic effects of l(1)Nts1 are autonomously expressed. © 1978.