AMNIOTIC-FLUID URIC-ACID LEVELS DETERMINED BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY WITH SPECTROPHOTOMETRIC AND ELECTROCHEMICAL DETECTION

A rapid and precise, reversed-phase high-performance liquid chromatographic method for amniotic fluid uric acid is described. Detection of uric acid and other naturally occurring constituents is based on UV absorption at a wavelength of 280 nm and direct electrochemical oxidation at a potential of +0.800 V. The total analysis time is short (20 min) and the assay requires only filtration of the samples. Uric acid levels were determined in 14 samples of amniotic fluid obtained during the 15th to 24th week of gestation. Results ranged from 0.897 to 4.39 mg per 100 ml of amniotic fluid. © 1979.