INDUCTION OF ALDOSE REDUCTASE AND POLYOL DEHYDROGENASE-ACTIVITIES IN AUREOBASIDIUM-PULLULANS BY D-XYLOSE, L-ARABINOSE AND D-GALACTOSE

The induction of aldose reductase and polyol dehydrogenase activities by D-xylose, L-arabinose, D-galactose and D-glucose was studied in the yeast-like organism Aureobasidium pullulans CCY 27-1-26. D-xylose and L-arabinose induced two distinct NADPH-dependent aldose reductases and the inducing saccharide was simultaneously the most efficient substrate for the corresponding enzymatic reaction. Polyol dehydrogenase induced by D-xylose, L-arabinose and D-galactose was strictly NAD+ -dependent and required only xylitol as a substrate of the enzymatic reaction. L-Arabitol did not act as a substrate for L-arabinose-induced polyol dehydrogenase either in the presence of NAD+ or NADP+.