DIRECT INVIVO GENE-TRANSFER TO EPENDYMAL CELLS IN THE CENTRAL-NERVOUS-SYSTEM USING RECOMBINANT ADENOVIRUS VECTORS

被引：339

作者：

BAJOCCHI, G

FELDMAN, SH

CRYSTAL, RG

MASTRANGELI, A

机构：

[1] NHLBI, PULM BRANCH, BETHESDA, MD 20892 USA

[2] NHLBI, LAB ANIM MED & SURG SECT, BETHESDA, MD 20892 USA

来源：

NATURE GENETICS | 1993年 / 3卷 / 03期

关键词：

D O I：

10.1038/ng0393-229

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To evaluate the potential for adenovirus-mediated central nervous system (CNS) gene transfer, the replication deficient recombinant adenovirus vectors Ad.RSVbetagal (coding for beta-galactosidase) and Ad-alpha1AT (coding for human alpha1-antitrypsin) were administered to the lateral ventricle of rats. Ad.RSVbetagal transferred beta-galactosidase to ependymal cells lining the ventricles whereas Ad-alpha1AT mediated alpha1-antitrypsin secretion into the cerebral spinal fluid for 1 week. These observations, together with beta-galactosidase activity in the globus pallidus and substantia nigra following stereotactic administration of Ad.RSVbetagal to the globus pallidus, suggest that adenovirus vectors will be useful for CNS gene therapy.

引用

页码：229 / 234

页数：6

共 31 条

[1] GENE-TRANSFER INTO MAMMALIAN CENTRAL-NERVOUS-SYSTEM USING HERPES-VIRUS VECTORS - EXTENDED EXPRESSION OF BACTERIAL LACZ IN NEURONS USING THE NEURON-SPECIFIC ENOLASE PROMOTER [J].