A SEQUENCE-SPECIFIC DNA-BINDING PROTEIN RECOGNIZING A GA-RICH ELEMENT COOPERATES WITH OCT-1 AT THE HERPES-SIMPLEX VIRUS TYPE-1 IE3 PROMOTER

被引：8

作者：

BAILEY, AC ^{[1
]}

THOMPSON, R ^{[1
]}

机构：

[1] UNIV GLASGOW, DEPT VIROL, GLASGOW G11 5JR, SCOTLAND

来源：

INTERVIROLOGY | 1992年 / 34卷 / 02期

关键词：

HSV-1; TAATGARAT; IE GENE TRANSACTIVATION; GA-RICH ELEMENT;

D O I：

10.1159/000150265

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Herpes simplex virus immediate early (IE) gene transcription is regulated via the upstream TAATGARAT motif and involves the recruitment of the ubiquitous octamer-binding protein Oct-1 and a component of the virion Vmw65 into a multiprotein complex termed the immediate early complex (IEC). An upstream GA-rich element (GA-RE) has been postulated to mediate a response to Vmw65 independent of TAATGARAT. We have studied IEC formation in gel shift assays using DNA fragments containing these sequence elements from the HSV-1 IE gene 3 promoter in different combinations. The results show that a factor which footprints to the GA-RE cooperates with Oct-1 to increase IEC levels on a TAATGARAT motif located 5 bp 5' to the GA-RE. The factor alone is unable to promote IEC formation. We were unable to demonstrate any effect of this factor on a TAATGARAT motif located 16 bp from the GA-RE, suggesting that this effect is not universal for all TAATGARAT motifs.

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页码：74 / 85

页数：12

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