CDNA CLONING AND SEQUENCE-ANALYSIS OF THE 3'-TERMINAL REGION OF ZUCCHINI YELLOW MOSAIC-VIRUS RNA

被引:33
作者
GRUMET, R
FANG, GW
机构
[1] Department of Horticulture, Michigan State University, East Lansing
关键词
D O I
10.1099/0022-1317-71-7-1619
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The 3' half of the RNA of the cucurbit potyvirus zucchini yellow mosaic virus (ZMYV) was genetically cloned and the cDNA sequence of a portion of the putative RNA polymerase gene, the complete coat protein gene and the 3' untranslated region was determined. The coat protein and putative polymerase genes are both part of a continuous open reading frame as is the case for other potyviruses whose genomes are expressed as polyproteins. The Q/S protease cleavage site for the N terminus of the coat protein was deduced by alignment of the coat protein and polymerase genes with other potyviral sequences. The resulting protein has 279 amino acids and a calculated M(r) of 31,214. The predicted amino acid sequence indicates a ZYMV-unique N-terminal region and potyvirus-characteristic central and C-terminal regions. These data also verify that ZYMV is distinct from the cucurbit potyvirus watermelon mosaic virus 2.
引用
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页码:1619 / 1622
页数:4
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