DIFFERENTIAL EXPRESSION OF ALLOANTIGENS OF THE MAJOR HISTOCOMPATIBILITY COMPLEX ON UNFERTILIZED AND FERTILIZED MOUSE EGGS

被引:15
作者
HEYNER, S
HUNZIKER, RD
机构
[1] Department of Biological Sciences, Philadelphia College of Pharmacy and Science
来源
DEVELOPMENTAL GENETICS | 1979年 / 1卷 / 01期
关键词
cleavage stage embryos; immunofluorescence; major histocompatibility complex; oocytes;
D O I
10.1002/dvg.1020010108
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Mouse oocytes at the dictyate and metaphase II stages as well as fertilized eggs have been studied by indirect immunofluorescence for the expression of H‐2 histocompatibility antigens on surface membranes. Serologically specific reactivity to H‐2 antibody was observed as patchy fluorescence distributed over the surface of the oocyte membrane. In contrast, one‐cell zygotes exhibited variable reactivity, and early two‐cell stages were negative. Absorption studies confirmed the serologic specificity of the reactivity on oocytes, which could be shown to be due to H‐2 antibody. The results suggest that fertilization results in altered expression of major histocompatibility complex surface antigens, and confirms earlier studies that cleavage stage mouse embryos are not reactive with H‐2 antibody. Copyright © 1979 Wiley‐Liss, Inc.
引用
收藏
页码:69 / 76
页数:8
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