CONTROL OF AN AFFINITY PURIFICATION PROCEDURE USING A THERMAL BIOSENSOR

被引：2

作者：

FLYGARE, L ^{[1
]}

LARSSON, PO ^{[1
]}

DANIELSSON, B ^{[1
]}

机构：

[1] UNIV LUND,BOX 124,S-22100 LUND,SWEDEN

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1990年 / 36卷 / 07期

关键词：

D O I：

10.1002/bit.260360710

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lactate dehydrogenase (LDH) was recovered from a solution by affinity binding to an N6‐(6‐aminohexyl)‐AMP‐Sepharose gel. An enzyme thermistor unit was employed to continously measure the activity of the unbound LDH. The enzyme activity signal from the enzyme thermistor was used in a PID controller to regulate the addition of AMP‐Sepharose gel to the LDH solution. In another type of experiment, a desktop computer was utilized to control the addition of the adsorbent. Both systems worked satisfactorily, and enabled a rapid and accurate assessment of correct addition of adsorbent. Copyright © 1990 John Wiley & Sons, Inc.

引用

页码：723 / 726

页数：4

共 6 条

[1] EVALUATION OF THE ENZYME THERMISTOR AS A SPECIFIC DETECTOR FOR CHROMATOGRAPHIC PROCEDURES [J].