TYROSINATION STATE OF FREE TUBULIN SUBUNITS AND TUBULIN DISASSEMBLED FROM MICROTUBULES OF RAT-BRAIN TISSUE

被引：31

作者：

RODRIGUEZ, JA

BORISY, GG

机构：

[1] Laboratory of Molecular Biology, University of Wisconsin, Madison

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 89卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91862-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The content of endogenous COOH-terminal tyrosine in unpolymerized tubulin subunits from the brain extracts of new-born rats was twice as high as that of tubulin isolated from disassembled native microtubules. It was also observed that the proportion of free sites in tubulin from in vitro incorporation of tyrosine at the COOH-terminus in unpolymerized protein was about one-half of that obtained for the protein from disassembled microtubules. In the extracts of young adult rats the value for the endogenous COOH-terminal tyrosine content in unpolymerized subunits decreased and almost equaled the value for the polymerized tubulin which remained virtually constant. © 1979.

引用

页码：893 / 899

页数：7

共 11 条

[1] CAPABILITY OF TUBULIN AND MICROTUBULES TO INCORPORATE AND TO RELEASE TYROSINE AND PHENYLALANINE AND EFFECT OF INCORPORATION OF THESE AMINO-ACIDS ON TUBULIN ASSEMBLY [J].