THEOPHYLLINE ANTAGONIZES SOME EFFECTS OF PURINES IN THE INTESTINE BUT NOT THOSE OF INTRAMURAL INHIBITORY NERVE-STIMULATION

Hyoscine‐ and guanethidine‐treated preparations of longitudinal muscle of rabbit duodenum, guinea‐pig taenia caeci and fundic strip relaxed when exposed to noradrenaline, adenosine, adenosine triphosphate (ATP) or to field stimulation of their intramural nerves. In guinea‐pig taenia caeci and fundus, theophylline 100 μmol/l had no effect on responses to noradrenaline, adenosine, ATP and intramural nerve stimulation. In rabbit duodenum, theophylline 100 μmol/l antagonized some responses to adenosine but had no effect on responses to noradrenaline, ATP and intramural nerve stimulation. Theophylline 1 mmol/l itself relaxed the intestinal tissues and in the fundic strip and taenia caeci, these relaxant effects were associated with abolition of spike activity and cellular hyperpolarization. In the taenia caeci, the amplitude of inhibitory post‐junctional potentials was reduced. Theophylline 1 mmol/l antagonized the twitch suppression produced by adenosine and ATP in the transmurally‐stimulated guinea‐pig ileum but not that evoked by noradrenaline. It is concluded that theophylline can selectively antagonize some actions of purines in the intestine but that it does not specifically antagonize the effects of intramural inhibitory nerve stimulation. 1979 British Pharmacological Society