GENUS-SPECIFIC DETECTION OF SALMONELLAE USING THE POLYMERASE CHAIN-REACTION (PCR)

被引：59

作者：

COHEN, ND

NEIBERGS, HL

MCGRUDER, ED

WHITFORD, HW

BEHLE, RW

RAY, PM

HARGIS, BM

机构：

[1] Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station

[2] Department of Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station

[3] Texas Veterinary Medical Diagnostic Laboratory, Texas A&M University, College Station

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1993年 / 5卷 / 03期

关键词：

D O I：

10.1177/104063879300500311

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Oligonucleotide primers for the polymerase chain reaction (PCR) that enable genus-specific detection of members of the genus Salmonella were developed. The primers amplify a 496-bp genetic sequence of members of the genus Salmonella. Amplification of DNA extracted from all other genera of the family Enterobacteriaceae and various other gram-positive aerobic and anaerobic bacteria yielded negative results. Applications of the PCR using these genus-specific primers are discussed.

引用

页码：368 / 371

页数：4

共 17 条

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