ALTERED TACHYKININ EXPRESSION BY DORSAL-ROOT GANGLION NEURONS IN A RAT MODEL OF NEUROPATHIC PAIN

被引：67

作者：

MARCHAND, JE ^{[1
]}

WURM, WH ^{[1
]}

KATO, T ^{[1
]}

KREAM, RM ^{[1
]}

机构：

[1] TUFTS UNIV, NEW ENGLAND MED CTR, SCH MED, DEPT ANESTHESIOL, BOSTON, MA 02111 USA

来源：

PAIN | 1994年 / 58卷 / 02期

关键词：

CAUSALGIA; HYPERALGESIA; DORSAL ROOT GANGLIA; SUBSTANCE P; PREPROTACHYKININ; IN SITU HYBRIDIZATION HISTOCHEMISTRY;

D O I：

10.1016/0304-3959(94)90202-X

中图分类号：

R614 [麻醉学];

学科分类号：

100217 ;

摘要：

The experiments described in the present study approached nerve injury from both a biochemical and anatomical perspective by monitoring changes in expression of preprotachykinin (PPT) mRNA encoding the prototypic tachykinin substance P and related peptide species in neurons of the rat dorsal root ganglia (DRG) following unilateral chronic constriction injury of the sciatic nerve. In situ hybridization histochemistry (ISHH) analyses in conjunction with computer-assisted image processing were employed to quantify levels of PPT mRNA distributed in DRG neurons. Injury-induced changes in PPT mRNA expression by affected DRG neurons included: (1) at early postoperative times, generally increased levels of PPT mRNA associated with small and intermediate-size B cells exhibiting normal morphology, (2) at late postoperative times, markedly decreased levels of PPT mRNA associated with degenerating B cells, and (3) induction of PPT gene expression by large A cells which is highly correlated with degenerative morphological changes. The significant aspects of these changes are discussed with special emphasis on the contribution of altered transmitter expression by DRG neurons to the pathophysiology of causalgia. In particular, the induction of PPT gene expression by many of the large neurons undergoing degenerative changes may represent an important biochemical parameter which is associated with the development and persistence of experimental allodynia.

引用

页码：219 / 231

页数：13

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