A COLORIMETRIC ASSAY FOR THE QUANTIFICATION OF BROOK TROUT (SALVELINUS-FONTINALIS) LYMPHOCYTE MITOGENESIS

被引：34

作者：

DALY, JG ^{[1
]}

MOORE, AR ^{[1
]}

OLIVIER, G ^{[1
]}

机构：

[1] FISHERIES & OCEANS CANADA,HALIFAX FISHERIES RES LAB,HALIFAX,NS B3J 2S7,CANADA

来源：

FISH & SHELLFISH IMMUNOLOGY | 1995年 / 5卷 / 04期

关键词：

MTT; LYMPHOCYTES; MITOGENS; SALVELINUS FONTINALIS; SALMONID; AEROMONAS SALMONICIDA;

D O I：

10.1006/fsim.1995.0026

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

A colorimetric assay using the dye MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) was developed to quantify the proliferative responses of break trout (Salvelinus fontinalis) peripheral blood and kidney lymphocytes. Mitogenic responses were assayed using the mitogens LPS, PHA, ConA and formalin-killed Aeromonas salmonicida. Optimal mitogenesis occurred only in the presence of homologous plasma. The assay proved to be a simple and reproducible alternative to the traditional H-3-thymidine incorporation assay.

引用

页码：265 / 273

页数：9

共 18 条

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