DETERMINATION OF FLUORIDE IN FEED MIXTURES BY CAPILLARY ISOTACHOPHORESIS

被引:8
作者
BLATNY, P
KVASNICKA, F
机构
[1] Department of Carbohydrate Chemistry and Technology, Institute of Chemical Technology Prague, CZ-166 28 Prague 6
关键词
D O I
10.1016/0021-9673(94)80298-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An isotachophoretic (ITP) method for the determination of fluoride in feed mixtures was developed. A sample of feed mixture, after extraction with 1 M HCl, was analysed using a ZKI 02 column-coupling isotachopherograph. Leading electrolytes for presentation and analytical capillaries consisted of 0.008 M HCl-0.022 M epsilon-aminocaproic acid (EACA)-0.001 M CaCl2-0.05% hydroxypropylmethyl cellulose (HPMC) and 0.002 M HCl-0.005 M EACA-0.05% HPMC, respectively. The terminating electrolyte was 0.01 M tartaric acid. The fluoride released from samples by microdiffusion in 25% perchloric acid was determined using an Ionosep 900.1 single capillary isotachopherograph with 0.002 M HCl-0.005 M EACA-0.05% HPMC as the leading electrolyte and 0.002 M tartaric acid as the terminating electrolyte. The detection limit, depending on the sample treatment, was as low as 4 mu g/g as fluoride. A comparison of the developed ITP method with ion-selective electrode method was carried out.
引用
收藏
页码:223 / 228
页数:6
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