CHARACTERIZATION OF THIOBARBITURIC ACID REACTIVITY IN HUMAN-PLASMA AND URINE

被引：119

作者：

GUTTERIDGE, JMC

TICKNER, TR

机构：

[1] Department of Clinical Biochemistry, Whittington Hospital, London

来源：

ANALYTICAL BIOCHEMISTRY | 1978年 / 91卷 / 01期

关键词：

D O I：

10.1016/0003-2697(78)90838-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The specificity of the thiobarbituric acid reaction (TBA) has been investigated using techniques of high-performance thin-layer chromatography and spectrofluorimetry. It was found that malondialdehyde (MDA) derived from different lipid and nonlipid origins formed the same MDA-TBA complex. This complex could be separated from other TBA-reactive compounds by both chromatography and spectrofluorimetry. Normal human plasma and urine both formed an MDA-TBA complex along with other TBA-reactive compounds. In plasma this was associated mainly with phosphatidylcholine and appeared to be peroxidic in reaction. Urine, however, contained polar MDA-forming compounds probably resulting from the oxidation of 2-deoxyaldoses during the acid-heating stage of the TBA test. © 1978.

引用

页码：250 / 257

页数：8

共 21 条

[1]

NOVEL INTERFERENCE IN THIOBARBITURIC ACID ASSAY FOR LIPID PEROXIDATION [J].