INTERFERON-GAMMA BINDS TO HEPARAN-SULFATE BY A CLUSTER OF AMINO-ACIDS LOCATED IN THE C-TERMINAL PART OF THE MOLECULE

被引：82

作者：

LORTAT-JACOB, H

GRIMAUD, JA

机构：

[1] Laboratoire de Pathologie Cellulaire, CNRS URA 602, Institut Pasteur, 69007 Lyon, Avenue Tony Garnier

来源：

FEBS LETTERS | 1991年 / 280卷 / 01期

关键词：

INTERFERON-GAMMA; HEPARAN SULFATE; HEPARIN; BINDING SITE; MOLECULAR STABILITY;

D O I：

10.1016/0014-5793(91)80225-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using three different approaches (domain mapping with monoclonal antibodies, limited enzymatic digestion and competition with synthetic peptides), we demonstrated that a cluster of basic amino acids on interferon-gamma is involved in its binding to heparan sulfate. This cluster (Lys125-Arg131) is localized in the C-terminal part of IFN-gamma. Once bound to heparan sulfate, IFN-gamma is protected against protease attack.

引用

页码：152 / 154

页数：3

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