STUDIES ON THE PROCESSIVITY OF HIGHLY PURIFIED CALF THYMUS 8S AND 7.3S DNA POLYMERASE-ALPHA

被引：10

作者：

MCKUNE, K

HOLMES, AM

机构：

[1] Biochemistry Department, Strathclyde University, The Todd Centre, Glasgow, G4 0NR Scotland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91907-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Template-challenge experiments indicate no gross difference in processivity of the calf thymus DNA polymerase .alpha. A and C enzymes. Both enzymes appear to be distributive. Results showing the apparent processive nature of both enzymes on poly (dC).cntdot.oligo (dG)10 when challenged with poly (dA).cntdot.oligo (dT)10 are explicable by the failure of both enzymes to bind to the challenging template rather than by the presence of an initiation factor which preferentially binds to certain templates.

引用

页码：864 / 870

页数：7

共 7 条

[1]

BAMBARA RA, 1978, J BIOL CHEM, V253, P413

[2]

BOLLUM FJ, 1966, PROCEDURES NUCLEIC A, P577

[3] DISTRIBUTIVE NATURE OF ENZYMATIC DNA-SYNTHESIS [J].