SEPARATION AND CHARACTERIZATION OF VENOM COMPONENTS IN LOXOSCELES-RECLUSA .2. PROTEASE ENZYME-ACTIVITY

被引：26

作者：

JONG, YS ^{[1
]}

NORMENT, BR ^{[1
]}

HEITZ, JR ^{[1
]}

机构：

[1] MISSISSIPPI STATE UNIV, MISSISSIPPI AGR & FORESTRY EXPT STN, DEPT ENTOMOL, STATE COLL, MS 39762 USA

来源：

TOXICON | 1979年 / 17卷 / 06期

关键词：

D O I：

10.1016/0041-0101(79)90227-7

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A protease from brown recluse spider venom hydrolyzes the amide linkage of amino acids containing aliphatic, aromatic, or basic side chains. The mol. wt of the enzyme was 29,600 by SDS electrophoresis. The Km for l-leucyl-β-naphthylamide is 3·75 × 10-4 M and the activation energy was 49,000 cal/mole. The pH profile indicates that the enzyme has a narrow active region centered at pH 7·2. Spider venom protease is inhibited irreversibly by ethylenediamine tetraacetic acid, N-ethylmaleimide and fluorescein mercuric acetate. © 1979.

引用

页码：529 / 537

页数：9

共 18 条

[1]

DIXON M, 1964, ENZYMES, P145

[2] PHYSIOLOGICAL ACTION OF LOXOSCELES-RECLUSA (G AND M) VENOM ON INSECT LARVAE [J].