IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION FOR THE ANALYSIS OF OXALATE IN FUNGAL CULTURE MEDIA

被引：12

作者：

DUTTON, MV ^{[1
]}

RASTALL, RA ^{[1
]}

EVANS, CS ^{[1
]}

机构：

[1] POLYTECH CENT LONDON,SCH BIOL & HLTH SCI,115 NEW CAVENDISH ST,LONDON W1M 8JS,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 587卷 / 02期

关键词：

D O I：

10.1016/0021-9673(91)85171-B

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid method to determine oxalate concentrations in liquid culture media of wood-rotting fungi has been developed using reversed-phase high-performance liquid chromatography. Separation was achieved on a Shandon Hypercarb column using 0.2 M orthophosphoric acid as the mobile phase, oxalate was detected at 210 nm. Oxalate was well resolved from other components in the culture medium, this enabled an accurate quantification of oxalate concentration in the range of 0.005-2 mM.

引用

页码：297 / 299

页数：3

共 13 条

[1]

CORCIA AD, 1982, CLIN CHEM, V28, P1457

[2] GAS-CHROMATOGRAPHIC SEPARATION OF ORGANIC-ACIDS AND ELECTROPHORETIC DETERMINATION OF PHOSPHATASES FROM VA MYCORRHIZAL ROOTS [J].

FABIG, B ;

VIELHAUER, K ;

MOAWAD, AM ;

ACHTNICH, W .

ZEITSCHRIFT FUR PFLANZENERNAHRUNG UND BODENKUNDE, 1989, 152 (02) :261-265

[3] LARGE SCALE PRODUCTION AND PURIFICATION OF LACCASE FROM CULTURES OF FUNGUS POLYPORUS VERSICOLOR AND SOME PROPERTIES OF LACCASE A [J].

FAHRAEUS, G ;

REINHAMMAR, B .

ACTA CHEMICA SCANDINAVICA, 1967, 21 (09) :2367-+

[4] SEPARATION OF ANIONIC AND CATIONIC COMPOUNDS OF BIOMEDICAL INTEREST BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON POROUS GRAPHITIC CARBON [J].

GU, GH ;

LIM, CK .

JOURNAL OF CHROMATOGRAPHY, 1990, 515 :183-192

[5]

HEINZ F, 1984, METHOD ENZYMAT AN, V6, P649

[6]

JAKOBY WB, 1962, METHOD ENZYMOL, V5, P637

[7] DETERMINATION OF URINARY OXALATE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY MONITORING WITH AN ULTRAVIOLET DETECTOR [J].

KATAOKA, K ;

TAKADA, M ;

KATO, Y ;

IGUCHI, M ;

KOHRI, K ;

KURITA, T .

UROLOGICAL RESEARCH, 1990, 18 (01) :25-28

[8]

LAKER MF, 1980, CLIN CHEM, V26, P827

[9]

LARSSON L, 1982, CLIN CHEM, V28, P2272

[10] CAPILLARY GAS-CHROMATOGRAPHY OF SOME MONOCARBOXYLIC, DICARBOXYLIC AND TRICARBOXYLIC-ACID ISOBUTYL ESTER DERIVATIVES [J].

MACKENZIE, SL ;

TENASCHUK, D ;

FORTIER, G .

JOURNAL OF CHROMATOGRAPHY, 1986, 367 (01) :181-186

← 1 2 →