ZETA-POTENTIAL OF HUMAN X-BEARING AND Y-BEARING SPERM

被引：47

作者：

ISHIJIMA, SA ^{[1
]}

OKUNO, M ^{[1
]}

MOHRI, H ^{[1
]}

机构：

[1] UNIV AIR, CHIBA 260, JAPAN

来源：

INTERNATIONAL JOURNAL OF ANDROLOGY | 1991年 / 14卷 / 05期

关键词：

HUMAN SPERM; X-BEARING SPERM; Y-BEARING SPERM; ZETA-POTENTIAL;

D O I：

10.1111/j.1365-2605.1991.tb01102.x

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

The zeta potential of human X- and Y-bearing sperm was measured by two different methods: (i) using an electrophoretic light scattering spectrophotometer, and (ii) using a laser-rotating prism. The X- and Y-bearing sperm were separated by free-flow electrophoresis, and their purities were determined by staining for the F-body using quinacrine mustard. The zeta potential of the sperm in the fraction containing more than 80% Y-bearing sperm was approximately -16 mV, whereas that of sperm in the fraction containing more than 95% X-bearing sperm was approximately -20 mV. In other words, the net negative-charge on the cell surface of human X-bearing sperm is higher than that of Y-bearing sperm.

引用

页码：340 / 347

页数：8

共 16 条

[1] ELECTROPHORETIC CHARACTERISTICS OF RAM AND RABBIT SPERMATOZOA [J].