ELEVATED N-MYRISTOYL TRANSFERASE-ACTIVITY IS REVERSED BY SODIUM ORTHOVANADATE IN STREPTOZOTOCIN-INDUCED DIABETIC RAT

被引：23

作者：

KING, MJ

PUGAZHENTHI, S

KHANDELWAL, RL

SHARMA, RK

机构：

[1] UNIV SASKATCHEWAN,ROYAL UNIV HOSP,DEPT BIOCHEM,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA

[2] UNIV SASKATCHEWAN,ROYAL UNIV HOSP,SASKATOON CANC CTR,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1165卷 / 03期

基金：

英国医学研究理事会;

关键词：

DIABETES; N-MYRISTOYL TRANSFERASE; VANADATE; (RAT LIVER);

D O I：

10.1016/0005-2760(93)90134-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-Myristoyl transferase (NMT) is the enzyme that covalently modifies several proteins important in signal transduction. Streptozotocin-induced diabetes resulted in a 2-fold increase in NMT activity from rat liver as compared to control animals. Administration of sodium orthovanadate to the diabetic rats reduced the activity of the NMT to 75-120% of the control values. Elevated NMT activity was observed with both cAMP-dependent protein kinase-derived and pp60src-derived peptide substrates. No significant change in the apparent K(m) was observed with the cAMP-dependent protein kinase-derived peptide substrate. Unlike in rat brain, in all conditions highest NMT activity was observed in the particulate fraction of rat liver.

引用

收藏

页码：259 / 262

页数：4

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