DIFFERENCE IN CHANGES OF MEMBRANE FLUIDITY OF POLYMORPHONUCLEAR LEUKOCYTES STIMULATED WITH PHORBOL-MYRISTATE ACETATE AND FORMYL-METHIONYL-LEUCYL-PHENYLALANINE - ROLE OF EXCITED OXYGEN SPECIES

被引：48

作者：

MASUDA, M ^{[1
]}

KOMIYAMA, Y ^{[1
]}

MURAKAMI, T ^{[1
]}

MURATA, K ^{[1
]}

HASUI, M ^{[1
]}

HIRABAYASHI, Y ^{[1
]}

KOBAYASHI, Y ^{[1
]}

机构：

[1] KANSAI MED UNIV,DEPT PEDIAT,MORIGUCHI,OSAKA 570,JAPAN

来源：

JOURNAL OF LEUKOCYTE BIOLOGY | 1990年 / 47卷 / 02期

关键词：

chronic granulomatous disease; Flow cytometry;

D O I：

10.1002/jlb.47.2.105

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Polymorphonuclear leukocytes (PMN) were stimulated with phorbol myristate acetate (PMA) and N-formyl-methionyl-leucyl phenylalanine (FMLP) to clarify the role of excited oxygen species in inducing changes of membrane fluidity. Membrane fluidity was assessed by the excimer-forming lipid technique using pyrenedecanoic acid and flow cytometry. Membrane fluidity of PMN decreased following stimulation with PMA, and the extent of decrease was both time-and dose-dependent. FMLP at 10-5M induced a decrease, while FMLP at 10-7M induced a rapid increase. On stimulation with 10-7M FMLP as well as in a resting condition, the change of membrane fluidity of PMN from patients with chronic granulomatous disease (CGD) was similar to that of normal PMN. However, on stimulation with PMA or 10-5M FMLP, CGD PMN did not show a significant decrease. In addition, normal PMN incubated with catalase inhibited the decrease. These findings suggest that the generation of excited oxygen species., particularly of H2O2, is important in inducing a decrease of PMN membrane fluidity.

引用

页码：105 / 110

页数：6

共 26 条

[1] ACTIVE OXYGEN SPECIES AND THE FUNCTIONS OF PHAGOCYTIC LEUKOCYTES [J].