A MR80K HEPATOCYTE SURFACE PROTEIN(S) INTERACTS WITH BASEMENT-MEMBRANE COMPONENTS

被引:16
作者
CLEMENT, B [1 ]
YAMADA, Y [1 ]
机构
[1] NIDR,DEV BIOL & ANOMALIES LAB,BETHESDA,MD 20892
关键词
D O I
10.1016/0014-4827(90)90098-U
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We have identified a Mmr 80K cell surface protein(s) from adult rat hepatocytes that binds basement membrane components, including collagen IV, heparan sulfate proteoglycan, and laminin. Freshly isolated hepatocytes were cell surface-labeled with 125I using the lactoperoxidase-catalyzed method, and detergent-solubilized membrane proteins were chromatographed on affinity columns prepared with purified basement membrane components. A Mr 80K protein was eluted with 0.15-1 M NaCl from a collagen IV column. Two proteins (Mr 80K and 38K) were eluted from a heparan sulfate proteoglycan column. The larger protein was also eluted from a column made with heparan sulfate side chains. Several proteins (Mr 80K, 67K, 45K, and 32K) bound to an affinity chromatography column made with the laminin A chain-derived synthetic peptide PA22-2, which is active for promoting cell attachment. When fractions eluted from these columns were analyzed by two-dimensional gel electrophoresis, the Mr 80K proteins showed similar patterns with a pI ranging from 8 to 9. The Mr 80K protein(s) may have an important role in the interaction of hepatocytes with basement membrane. © 1990.
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收藏
页码:320 / 323
页数:4
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