QUANTITATIVE FOOTPRINTING ANALYSIS - BINDING TO A SINGLE SITE

被引：21

作者：

REHFUSS, R ^{[1
]}

GOODISMAN, J ^{[1
]}

DABROWIAK, JC ^{[1
]}

机构：

[1] SYRACUSE UNIV,DEPT CHEM,SYRACUSE,NY 13244

来源：

BIOCHEMISTRY | 1990年 / 29卷 / 03期

关键词：

D O I：

10.1021/bi00455a027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The theory for measuring ligand binding constants from footprinting autoradiographic data associated with a single binding site is derived. If the ligand and DNA cleavage agent compete for a common site, the spot intensities are not proportional to the amount of DNA not blocked by ligand. The analysis of a single site is experimentally illustrated by using results for the anticancer drug actinomycin D interacting with the duplex d(TAGCGCTA)2 as probed with the hydrolytic enzyme DNase I. © 1990, American Chemical Society. All rights reserved.

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页码：777 / 781

页数：5

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