DETERMINATION OF COASH BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND ITS APPLICATION IN THE ASSAY OF LONG-CHAIN ACYL-COA DERIVATIVES

被引：42

作者：

INGEBRETSEN, OC

NORMANN, PT

FLATMARK, T

机构：

[1] Department of Biochemistry, University of Bergen, N-5000 Bergen

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 96卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90571-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic procedure is described for the determination of picomole amounts of CoASH, using a microparticulate, strong anion-exchange resin. The method was applied in a systematic study to optimize the conditions for alkaline hydrolysis of palmitoyl-CoA. The procedure, which ensures 100% recovery of CoASH by hydrolysis of paimitoyl-CoA, was found to be convenient also for the assay of the endogenous content of long-chain acyl-CoA derivatives in biological material. © 1979.

引用

页码：181 / 188

页数：8

共 28 条

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