2-DIMENSIONAL CRYSTALLIZATION OF REACTION CENTERS FROM CHLOROFLEXUS-AURANTIACUS

被引：3

作者：

BARNAKOV, AN

DEMIN, VV

KUZIN, AP

ZARGAROV, AA

ZOLOTAREV, AS

ABDULAEV, NG

机构：

[1] Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, 117871 GSP Moscow, V-437

来源：

FEBS LETTERS | 1990年 / 265卷 / 1-2期

关键词：

Electron microscopy; Image processing; Reaction center; Two-dimensional crystal;

D O I：

10.1016/0014-5793(90)80900-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two-dimensional crystals of photosynthetic reaction centers from Chloroflexus aurantiacus were obtained from protein-lipid-detergent micelles by detergent dialysis. The size of crystals was up to 2 μm. Some of them were multilayered crystals. However, other crystal forms were also observed. Preliminary image processing analysis showed that crystals of one crystal form referred to two-sided plane group p2 and had the following unit cell parameters: a = 17,6 nm, b = 18,0 nm, γ = 84°. The contour map of the crystal stain-excluding region was calculated by the Fourier-filtering procedure at about 2 nm resolution. © 1990.

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页码：126 / 128

页数：3

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