HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF S-ADENOSYLMETHIONINE AND S-ADENOSYLHOMOCYSTEINE IN RAT-LIVER

A sensitive and precise high performance liquid chromatographic method for the simultaneous quantitation of S-adenosylmethio-nine (SAMe) and S—adenosylhomocysteine (SAH) in rat liver is de-scribed. Liver is extracted by homogenization in 1,5 M PCA. SAMe and SAH are purified from contaminating substances by chromatography on Dow ex 50 W x 2 and analyzed by HPLC on conventional styrene-ty-pe anion exchanger. Analytical chromatography, performed under isocratic elution conditions, is achieved in 18 min. SAH is determined as such while SAMe as adenine after hydrolysis at 60° C, un der alkaline conditions. The sensitivity of the analytical proce dure allows the determination of SAMe and SAH in less than 200 mg of liver sample. © 1979, Taylor & Francis Group, LLC. All rights reserved.