MICRO INVERSE-DETECTION - A POWERFUL TECHNIQUE FOR NATURAL PRODUCT STRUCTURE ELUCIDATION

Sample quantities required for heteronuclear correlation spectra are significantly reduced when the experiments are performed in a micro-inverse detection probe. Spectra are reported for a 12-mu-g sample of cryptolepine HCl [1] dissolved in 145-mu-l of DMSO-d6. HMQC data shown were recorded in 16 h; acceptable quality data can be acquired in 8 h at this level Excellent quality HMBC data were acquired on a 35-mu-g sample in 21 h.