CHLORIDE CHANNELS AND ANION FLUXES IN A HUMAN COLONIC EPITHELIUM (HCA-7)

1 Colonic epithelial cells, derived from a human adenocarcinoma (HCA-7), were examined by the patch clamp technique. 2 Outwardly rectifying anion (Cl-) channels were identified in the apical membrane. The conductance was g(in) almost-equal-to 26 pS, g(out) almost-equal-to 40 pS. The open state probability of the channels increased with depolarization and the selectivity for Cl- over K+ (P(Cl)/P(K)) was almost-equal-to 7.5. 3 The channels were sensitive to intracellular adenosine 3':5'-cyclic monophosphate (cyclic AMP, 0.1 mm), but not to Ca2+ (at concentrations up to 1 mm). At depolarized potentials the channels were blocked by pirentanide (1-5-mu-M) applied intracellularly. 4 HCA-7 monolayers loaded with I-125- (as a marker for Cl-) were used to measure I- efflux and converted to instantaneous rate constants. 5 The rate constant for I- efflux was increased by forskolin and lysylbradykinin (LBK). The effects of forskolin were not effected by BAPTA (an intracellular calcium chelator). The effects of LBK were inhibited by BAPTA and by Ba2+, indicating that LBK raised intracellular Ca2+ (Ca(i)) which activates Ca2+-sensitive K-channels, the latter being blocked by Ba2+. 6 Although it cannot be conclusively proved that the outwardly rectifying chloride channels described here are solely or partially responsible for the increased anion efflux or transepithelial chloride secretion, the channels are likely to be more relevant for cyclic AMP-requiring rather than Ca2+-requiring secretagogues.