TITYUS BAHIENSIS TOXIN IV-5B SELECTIVELY AFFECTS NA CHANNEL INACTIVATION IN CHICK DORSAL-ROOT GANGLION NEURONS

被引:13
作者
TREQUATTRINI, C
ZAMUDIO, FZ
PETRIS, A
PRESTIPINO, G
POSSANI, LD
FRANCIOLINI, F
机构
[1] UNIV PERUGIA, DIPARTIMENTO BIOL CELLULARE & MOLEC, I-06100 PERUGIA, ITALY
[2] UNIV NACL AUTONOMA MEXICO, INST BIOTECHNOL, DEPT MOLEC RECOGNIT & STRUCT BIOL, CUERNAVACA 62271, MORELOS, MEXICO
[3] CNR, IST CIBERNET & BIOFIS, I-16146 GENOA, ITALY
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY | 1995年 / 112卷 / 01期
关键词
SCORPION TOXIN; SCORPION VENOM; TITYUS BAHIENSIS; DRG NEURONS; NA CHANNEL INACTIVATION; ION CHANNELS; NA CURRENTS; PATCH CLAMP; WHOLE-CELL; EMBRYONIC NEURONS;
D O I
10.1016/0300-9629(95)00096-P
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel toxin was isolated from the venom of the Brazilian scorpion Tityus (T,) bahiensis, The N-terminal amino acid sequence of this toxin was shown to be 80% identical to the corresponding segment of T, serrulatus toxin IV-5. The new toxin was thus named toxin IV-5b, Toxin IV-5b was found to markedly slow inactivation of Na channel in dorsal root ganglion neurons from chick embryo, By contrast, Na channel activation was only negligibly delayed, and deactivation completely unaffected, Similarly unaffected by the toxin were K and Ca currents, The slowing effect of the toxin starts to appear at concentrations of c. 80 nM, and shows a K-D of 143 nM, With a toxin concentration of 2.4 mu M, the Na channel inactivation time constant was increased c, 3-fold with respect to the control, The slowing of inactivation was voltage dependent, and increased with depolarization.
引用
收藏
页码:21 / 28
页数:8
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