FLUORESCENCE DEPOLARIZATION BY LIGHT-SCATTERING IN TURBID SOLUTIONS

被引：74

作者：

TEALE, FWJ

机构：

[1] Department of Biochemistry, University of Birmingham, Birmingham, Edgbaston

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1969年 / 10卷 / 06期

关键词：

D O I：

10.1111/j.1751-1097.1969.tb05701.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract— The effect of light‐scattering on the fluorescence polarization of solutions has been investigated both theoretically and empirically. The criteria by which turbidity depolarization may be recognised are described, and differentiated from those which characterise depolarization through optical rotation or birefringence. These criteria have been used to confirm that the fluorescence depolarization which accompanies actomyosin formation originates in increased light‐scattering rather than a real change in relaxation time. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：363 / &

共 15 条

[1] FLUORESCENCE POLARIZATION OF COMPLEXES OF 1-ANILINO-8-NAPHTHALENESULFONATE WITH BOVINE SERUM ALBUMIN . EVIDENCE FOR PREFERENTIAL ORIENTATION OF LIGAND [J].