A CDNA CLONE CLOSELY ASSOCIATED WITH NON-A-HEPATITIS, NON-B-HEPATITIS

被引:45
作者
MAENO, M
KAMINAKA, K
SUGIMOTO, H
ESUMI, M
HAYASHI, N
KOMATSU, K
ABE, K
SEKIGUCHI, S
YANO, M
MIZUNO, K
SHIKATA, T
机构
[1] HOKKAIDO RED CROSS BLOOD CTR,SAPPORO,JAPAN
[2] NATL INST HLTH,DEPT PATHOL,TOKYO 141,JAPAN
[3] NAGASAKI CHUO NATL HOSP,INST CLIN RES,NAGASAKI,JAPAN
[4] CHEMO SERO THERAPEUT RES INST,KUMAMOTO,JAPAN
关键词
D O I
10.1093/nar/18.9.2685
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A lambda gtl1 cDNA library was constructed from RNA purified from hepatitis B viral surface antigen-negative human plasma with high alanine aminotransferase activity. A cDNA clone, designated s C8-2, was isolated by immunoscreening with mixed sera from non-A, non-B hepatitis (NANBH) carrier and convalescent chimpanzees. The recombinant protein produced by C8-2 reacted specifically with sera of patients in the chronic phase of NANBH. The sequence of C8-2, 269 bp, did not hybridized with any human or chimpanzee genomic DNA, and had no homology with those of primates and viruses. The existence of this sequence in RNA of possibly infectious plasma was shown by RNA blot hybridization and by Southern blot analysis of products amplified by the polymerase chain reaction. These results strongly suggest that C8-2 is derived from the agent of this viral hepatitis. © 1990 Oxford University Press.
引用
收藏
页码:2685 / 2689
页数:5
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