MICRODETERMINATION OF SELENIUM IN PROTEIN-FRACTIONS ISOLATED BY ANALYTICAL METHODS

被引：10

作者：

VEZINA, D

BELANGER, R

BLEAU, G

机构：

[1] UNIV MONTREAL,DEPT OBSTET & GYNECOL,MONTREAL H3C 3J7,QUEBEC,CANADA

[2] MAISONNEUVE ROSEMONT MED CTR,MONTREAL H1T 2M4,QUEBEC,CANADA

[3] UNIV MONTREAL,DEPT HEMATOL,MONTREAL H3C 3J7,QUEBEC,CANADA

来源：

BIOLOGICAL TRACE ELEMENT RESEARCH | 1990年 / 24卷 / 02期

关键词：

2,3-diaminonaphthalene; fluorescence; glutathione-peroxidase; Selenium determination; selenoproteins;

D O I：

10.1007/BF02917203

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed for the determination of selenium associated with proteins in chromatographic fractions, in polyacrylamide gels, and on nitrocellulose membranes after transfer. This method involves the complete digestion of samples in the purest nitric and perchloric acids and takes advantage of the specificity afforded by the 99% pure 2,3-diaminonaphthalene. The use of these and other reagents of highest purity produces very low blank values and allows a detection limit as low as 0.76 picomoles (60 picograms) of selenium. Quantitative recoveries of selenium in glutathione peroxidase and low coefficients of variation were obtained. © 1990 The Humana Press Inc.

引用

页码：153 / 162

页数：10

共 10 条

[1] EVIDENCE FOR SPECIFIC SELENIUM TARGET TISSUES AND NEW BIOLOGICALLY IMPORTANT SELENOPROTEINS [J].