SITE SPECIFIC ENZYMATIC CLEAVAGE OF RNA

被引：357

作者：

DONISKELLER, H

机构：

[1] Department of Biochemistry and Molecular Biology, Harvard University, Cambridge

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/nar/7.1.179

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hybridization of a DNA oligonucleotide (a specific tetramer or longer) will direct a cleavage by RNase H (EC 3.1.4.34) to a specific site in RNA. The resulting fragments can then be labeled at their 5′ or 3′ ends, purified, and sequenced directly. This procedure is demonstrated with two RNA molecules of known sequence: 5.8S rRNA from yeast (158 nucleotides) and satellite tobacco necrosis virus (STNV) RNA (1240 nucleotides). © 1979 Information Retrieval Limited.

引用

页码：179 / 192

页数：14

共 32 条

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