THE USE OF THIOGLYCOLLATE TO DEMONSTRATE DNA AP (APURINIC APYRIMIDINIC-SITE) LYASE ACTIVITIES - BIOLOGICAL CONSEQUENCES OF THIOL ADDITION TO THE 5' PRODUCT OF A BETA-ELIMINATION REACTION AT AN AP SITE IN DNA

被引：3

作者：

BRICTEUXGREGOIRE, S ^{[1
]}

VERLY, WG ^{[1
]}

机构：

[1] UNIV LIEGE,FAC SCI,BIOCHIM LAB,B-4000 LIEGE 1,BELGIUM

来源：

BIOCHEMICAL JOURNAL | 1991年 / 273卷

关键词：

D O I：

10.1042/bj2730777

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thioglycollate reacts with the 5' product of AP lyase activity on apurinic/apyrimidinic (AP) sites in DNA. The 3'-terminal thioglycollate-unsaturated sugar 5-phosphate adduct can be released by the use of Escherichia coli endonuclease IV or endonuclease VI, and identified by DEAE-Sephadex chromatography. In contrast, the mammalian AP endonuclease is unable to excise a 3'-terminal thiol-unsaturated sugar adduct; this lesion, which must sometimes occur in vivo, might be irreparable and have pathological consequences.

引用

页码：777 / 782

页数：6

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