MUTAGENESIS OF THE GENE ENCODING AMICYANIN OF PARACOCCUS-DENITRIFICANS AND THE RESULTANT EFFECT ON METHYLAMINE OXIDATION

被引：96

作者：

VANSPANNING, RJM

WANSELL, CW

REIJNDERS, WNM

OLTMANN, LF

STOUTHAMER, AH

机构：

[1] Department of Microbiology, Biological Laboratory, 1081 HV Amsterdam

来源：

FEBS LETTERS | 1990年 / 275卷 / 1-2期

关键词：

Amicyanin; Electron transport chain; Gene replacement; Methylamine dehydrogenase; Paracoccus denitrificans;

D O I：

10.1016/0014-5793(90)81475-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene encoding the blue-copper protein amicyanin was isolated from a genomic bank of Pracoccus denitrificans by using a synthetic oligonucleotide. It is located directly downstream of the gene encoding the small subunit of methylmine dehydrogenase. Amicyanin is transcribed as a presursor protein with a signal sequence, typical for periplasmic proteins. Specific inactivation of amicyanin by means of gene replacement techniques resulted in the complete loss of the ability to grow on methylamine. © 1990.

引用

页码：217 / 220

页数：4

共 20 条

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