LEUKOCYTE CYTOSKELETAL STRUCTURE DETERMINES CAPILLARY PLUGGING AND NETWORK RESISTANCE

被引:38
作者
HARRIS, AG [1 ]
SKALAK, TC [1 ]
机构
[1] UNIV VIRGINIA, HLTH SCI CTR, DEPT BIOMED ENGN, BOX 377, CHARLOTTESVILLE, VA 22908 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 265卷 / 05期
关键词
MICROCIRCULATION; RHEOLOGY; HEMODYNAMICS; WHITE BLOOD CELLS; CYTOCHALASIN-D; CYTOSKELETON; LEUKOCYTE ACTIVATION; FLOW RESISTANCE;
D O I
10.1152/ajpheart.1993.265.5.H1670
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Leukocyte plugging in capillaries may play an important role in microvascular hemodynamics under pathological conditions that result in leukocyte activation. Previous studies have quantified the effects of transient capillary plugging on microvascular resistance for control and activated leukocytes, but have not focused on the intracellular mechanisms responsible. The frequency and duration of leukocyte-capillary plugging were measured throughout capillary networks in spinotrapezius muscle of anesthetized rats under normal physiological conditions and after leukocyte activation coupled with cytoskeletal F-actin depolymerization, using superfusion with 10(-7) M N-formyl-methionyl-leucyl-phenylalanine (FMLP) and 0.3 muM cytochalasin D (CD). These data were used to estimate the increase in microvascular flow resistance due to leukocyte plugging. The increase was 1.1 % in control and 0.7 % in the activated FMLP-CD state and was not significantly different. CD abolished the previously reported 16 % resistance increase caused by FMLP activation [Harris and Skalak, Am. J. Physiol. 264 (Heart Circ. Physiol. 31): H909-H916,1993.], suggesting that leukocyte plugging in capillaries is primarily determined by leukocyte cytoplasmic viscosity and is not significantly affected by cell adhesion. Thus F-actin-mediated cytoskeletal reorganization during leukocyte activation has significant impact on microvascular resistance.
引用
收藏
页码:H1670 / H1675
页数:6
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