GENETIC-ANALYSIS OF LOW M(R) GLUTENIN SUBUNITS FRACTIONATED BY 2-DIMENSIONAL ELECTROPHORESIS (A-PAGEXSDS-PAGE)

Alkylated glutenin subunits of F-7 progenies from the cross between the Italian bread wheat cultivar Costantino and the Canadian cultivar Neepawa were fractionated by one-dimensional A-PAGE and SDS-PAGE and by two-dimensional A-PAGE x SDS-PAGE. Each gliadin allele at the Gli-l loci of the parental cultivars was shown to be associated with a specific allele at each of the Glu-3 loci, at which low M(r) glutenin subunits are encoded. The Glu-A3 locus was found to code for two low M(r) subunits in Neepawa and three in Costantino. In this latter cultivar, eight low M(r) subunits were assigned to each of the Glu-B3 and Glu-D3 loci, whereas seven subunits were attributed to the Glu-B3 locus and seven to the Glu-D3 locus in Neepawa. A-PAGE x SDS-PAGE can be employed for a detailed description of low M(r) subunits of glutenin in different cultivars following a genetic approach based on the correspondence between the alleles at the Gli-1 and Glu-3 loci.