ULTRASTRUCTURAL-LOCALIZATION OF NADPH-DIAPHORASE AND COLOCALIZATION OF NITRIC-OXIDE SYNTHASE IN ENDOTHELIAL-CELLS OF THE RABBIT AORTA

被引：39

作者：

LOESCH, A

BELAI, A

BURNSTOCK, G

机构：

[1] UCL, DEPT ANAT & DEV BIOL, LONDON WC1E 6BT, ENGLAND

[2] UCL, CTR NEUROSCI, LONDON WC1E 6BT, ENGLAND

来源：

CELL AND TISSUE RESEARCH | 1993年 / 274卷 / 03期

关键词：

NADPH-DIAPHORASE; NITRIC OXIDE SYNTHASE; COLOCALIZATION; AORTIC ENDOTHELIUM; RABBIT;

D O I：

10.1007/BF00314551

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This is the first report on the ultrastructural pattern of distribution of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) in endothelial cells, using the rabbit aorta, and its colocalization with the neuronal isoform (type I) of nitric oxide synthase. About 30% of the endothelial cells showed a positive reaction for NADPH-d compared to about 6% for nitric oxide synthase immunoreactivity. Simultaneous double histochemical-immunocytochemical labelling procedures indicate that all of the cells displaying nitric oxide synthase-positive reactivity also contained NADPH-d; the remainder of NADPH-d-positive endothelial cells were negative for this isoform of nitric oxide synthase. Nitric oxide synthase-immunogold labelling was mostly associated with free ribosomes, while NADPH-d activity was distributed largely in patches in the cytoplasm and in association with the cell membrane.

引用

页码：539 / 545

页数：7

共 16 条

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