A RIBOSOMAL FRAMESHIFTING ERROR DURING TRANSLATION OF THE ARGI MESSENGER-RNA OF ESCHERICHIA-COLI

被引:30
作者
FU, CW [1 ]
PARKER, J [1 ]
机构
[1] SO ILLINOIS UNIV,DEPT MICROBIOL,CARBONDALE,IL 62901
来源
MOLECULAR & GENERAL GENETICS | 1994年 / 243卷 / 04期
关键词
CODON; FRAMESHIFTING; RIBOSOME; TRANSLATION;
D O I
10.1007/BF00280474
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using fusions between the Escherichia coli genes argI and lacZ, it has been demonstrated that ribosomal frameshifting occurs at a frequency of between 3% and 16% within the argl mRNA, soon after the initiation codon. The frameshift involves a phenylalanyl-tRNA shifting into the + 1 frame at the sequence UUU-U/C. The shift does not occur if the in-frame phenylalanine codon UUU is replaced by UUC. The level of frameshifting is higher in dense cultures and is not dependent on phenylalanine starvation. In the wild-type argI gene this frameshifting event would be an error, leading to a truncated, non-functional protein. Therefore, it is unlike the numerous examples of required frameshifting events that have been described in other genes.
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页码:434 / 441
页数:8
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