NATIVE ZONA-PELLUCIDA STRUCTURE IS REQUIRED FOR COMPLETION OF SPERM ACROSOME REACTION IN PORCINE FERTILIZATION

被引：9

作者：

YOSHIZAWA, M

NAGAI, T

YONEZAWA, N

NAKANO, M

机构：

[1] CHIBA UNIV,FAC SCI,DEPT CHEM,INAGE KU,CHIBA 263,JAPAN

[2] TOHOKU NATL AGR EXPTL STN,DEPT ANIM PROD,MORIOKA,IWATE 02001,JAPAN

来源：

THERIOGENOLOGY | 1994年 / 41卷 / 06期

关键词：

IN VITRO FERTILIZATION; ZONA PELLUCIDA; POLYSPERMY; ACROSOME REACTION;

D O I：

10.1016/0093-691X(94)90489-6

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In fertilization in vitro, the penetration rate of zona-intact porcine oocytes by cryopreserved epididymal spermatozoa was about 100% while that of zona-free oocytes was only 30%. Spermatozoa treated with calcium ionophore A23187 penetrated both and zona-free oocytes at the rate of more than 90%. of spermatozoa with solubilized porcine zonae pellucidae hardly induced acrosome reaction and did not increase the penetration rate. These results suggest that the structure of the zona is necessary for completion of acrosome reaction.

引用

页码：1307 / 1313

页数：7

共 13 条

[1] CAPACITATION OF RABBIT SPERMATOZOA INVITRO [J].