Cloning and characterisation of the rabbit 5-HT1D alpha and 5-HT1D beta receptors

被引：27

作者：

Harwood, G

Lockyer, M

Giles, H

Fairweather, N

机构：

[1] WELLCOME RES LABS,MOLEC PHARMACOL RES GRP,BECKENHAM BR3 3BS,KENT,ENGLAND

[2] WELLCOME RES LABS,RECEPTOR PHARMACOL RES GRP,BECKENHAM BR3 3BS,KENT,ENGLAND

来源：

FEBS LETTERS | 1995年 / 377卷 / 01期

关键词：

G-protein coupled receptor; serotonin; 5-hydroxytryptamine; rabbit;

D O I：

10.1016/0014-5793(95)01308-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genes encoding the rabbit 5HT(1D alpha) and 5HT(1D beta) receptors have been cloned, The deduced amino acid sequence of these receptors shows 91-92% amino acid sequence identity with their human homologues, and similar high sequence identity with homologues from other species. The receptors were transiently expressed in COS-7 cells and exhibit a pharmacological profile closely resembling their human homologues, including a higher affinity of ketanserin for the 5-HT1D alpha subtype. However, sumatriptan had a lower affinity for both the rabbit receptors compared to their human counterparts. This may be accounted for by differences between the primary amino acid sequences of these species homologues.

引用

页码：73 / 76

页数：4

共 17 条

[1] CLONING OF ANOTHER HUMAN SEROTONIN RECEPTOR (5-HT1F) - A 5TH 5-HT1 RECEPTOR SUBTYPE COUPLED TO THE INHIBITION OF ADENYLATE-CYCLASE [J].