MOLECULAR CLONING AND SEQUENCE-ANALYSIS OF ADULT CHICKEN BETA-GLOBIN CDNA

被引：91

作者：

RICHARDS, RI ^{[1
]}

SHINE, J ^{[1
]}

ULLRICH, A ^{[1
]}

WELLS, JRE ^{[1
]}

GOODMAN, HM ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,HOWARD HUGHES MED INST,DEPT BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 05期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/nar/7.5.1137

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The molecular cloning and nucleotide sequence analysis of adult chicken β globin mRNA is reported. DNA sequences derived from in vitro transcription of globin mRNA were purified and amplified as recombinant DNA using the plasmid pBR322. Sequence analysis of several clones coding for β globin strongly suggests that transcription errors may be generated near the 5′ end of transcripts in vitro by reverse transcription. The complete sequence of the longest β globin insert containing 51 bases of the 5′ untranslated region as well as the complete coding and 3′ untranslated regions has been determined. © 1979 Information Retrieval Limited.

引用

页码：1137 / 1146

页数：10

共 24 条

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