SIMPLE ISOCRATIC HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR MEASURING PYRIDOXINE KINASE-ACTIVITY IN CRUDE BIOLOGICAL EXTRACTS

被引:5
作者
ARGOUDELIS, CJ
机构
[1] Department of Food Science, University of Illinois, Urbana, IL 61801, 580 Bevier Hall
来源
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 526卷 / 01期
关键词
D O I
10.1016/S0378-4347(00)82480-9
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
To measure the enzymatic activity of pyridoxine kinase (EC 2.7.1.35) when pyridoxine or pyridoxamine are the substrates an additional enzymatic step is usually required. The products of the kinase activity, pyridoxine 5′-phosphate or pyridoxamine 5′-phosphate, are oxidized, enzymatically, to pyridoxal 5′-phosphate (co-enzyme) which is then measured either spectrophotometrically or using apo-enzymes. In this report the enzymatic activity of pyridoxine kinase, in crude biological extracts, is assayed by a simple high-performance liquid chromatographic method which determines the amount of pyridoxine 5′-phosphate formed when pyridoxine is the substrate. The same method could be used when pyridoxamine is the substrate. © 1990.
引用
收藏
页码:25 / 33
页数:9
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